Isotope tracing
Isotope tracing is planned for the metabolic process and experimental model. Suitability of the ¹⁸O-based approaches described in our service scope is assessed for each application.
FLUXOMICS
Fluxomics
Metabolite levels and metabolic flux provide different information. Fluxomics brings together tracing strategy, sampling design and data analysis to investigate pathway dynamics.
Discuss your project ↗Targeted metabolic flux
Target pathways, sampling times and required measurements are defined from the research question. Concentration data alone are not interpreted as flux measurements.
Interpreting metabolic dynamics
Interpret data in the context of metabolic turnover and pathways, considering the tracing approach and computational scope.
RESEARCH FOCUS
Let’s define the focus of your study.
PLAN YOUR STUDY
What will you provide and receive?
Research question
Compare isotope labelling and, where appropriate, plan model-based flux estimation.
Before sending samples
Share the tracer, medium, exposure duration, sampling times and experimental groups in advance.
Deliverables
Isotopologue tables, labelling plots and a method summary as agreed; modelling may be a separate work item.
Defining the scope
Labelling distributions are not direct flux rates. Data sufficiency and model requirements are evaluated for flux estimation.
EXAMPLE ANALYSIS OUTPUT
How is labelled carbon distributed?
An M+0–M+3 distribution for an example three-carbon metabolite illustrates isotope labelling under two conditions.
Possible outputs for your study
Isotopologue distribution · Labelling comparison · Flux modelling where in scope
HOW WE WORK
A process planned together.
- 01
Share your research
Research question, samples, study groups and replicates.
- 02
Define the scope
Sample preparation, analytical approach and reporting plan.
- 03
Review the results
Agreed analyses and scope of biological interpretation.
LET’S TALK SCIENCE
Let’s start with an email.
Share your research question, analysis or manufacturing requirements. Attach drawings and relevant files to your email.
DIRECT CONTACTbiomicscience@gmail.comÜniversiteler Mah. 1596 Cad. Teknokent Kuluçka Merkez Binası No: 6 C, Unit 11, Çankaya / Ankara, Türkiye